Saturday, January 19, 2008
kunstmatig hart
Functional heart grown in laboratory
14 January 2008
MedWire News: Scientists have grown an artificial, functioning heart in the laboratory by stripping myocardial cells from a full-sized adult cadaver heart and reseeding it with neonatal heart cells.
They hope the technology will eventually lead to the development of artificial hearts that can be used in transplant surgery.
"The idea would be to develop transplantable blood vessels or whole organs that are made from your own cells," said principal investigator Doris Taylor, from University of Minnesota in Minneapolis, USA.
Taylor's team sought to overcome the challenge of creating a three-dimensional scaffold that closely mimics the complex cardiac architecture using a technique called decellularization to remove cells from the heart, leaving the extracellular matrix intact to act as a "platform" on which to grow the artificial organ.
The researchers compared the degree of cell removal from both rat and pig cadaver hearts with different detergents. Antegrade coronary perfusion with a sodium dodecyl sulfate solution over 12 hours yielded a fully decellularized construct, they report in the journal Nature Medicine.
The researchers reseeded such constructs with rat neonatal cardiac cells and aortic endothelial cells in a bioreactor that recreated cardiac physiology, with simulated coronary perfusion.
The perfused organ culture was maintained for between 8 and 28 days. By day 8 after seeding, the heart constructs showed electrical and contractile responses to single paces.
Under physiological load, the heart constructs produced a contractile force of around 2.4 mmHg, equivalent to approximately 2% of adult rat heart function, and 25% of 16-week-old fetal human heart function.
The researchers are optimistic that the technology could help to increase the donor organ pool. The supply of donor hearts for end-stage heart failure patients to undergo heart transplantation is limited, and once patients receive a heart they face lifelong immunosuppression, often trading heart failure for hypertension, diabetes, and renal failure, they explain.
"We used immature heart cells in this version, as a proof of concept. We pretty much figured heart cells in a heart matrix had to work," Taylor commented. "Going forward, our goal is to use a patient's stem cells to build a new heart."
Nature Med 2008; Advance online publication
14 January 2008
MedWire News: Scientists have grown an artificial, functioning heart in the laboratory by stripping myocardial cells from a full-sized adult cadaver heart and reseeding it with neonatal heart cells.
They hope the technology will eventually lead to the development of artificial hearts that can be used in transplant surgery.
"The idea would be to develop transplantable blood vessels or whole organs that are made from your own cells," said principal investigator Doris Taylor, from University of Minnesota in Minneapolis, USA.
Taylor's team sought to overcome the challenge of creating a three-dimensional scaffold that closely mimics the complex cardiac architecture using a technique called decellularization to remove cells from the heart, leaving the extracellular matrix intact to act as a "platform" on which to grow the artificial organ.
The researchers compared the degree of cell removal from both rat and pig cadaver hearts with different detergents. Antegrade coronary perfusion with a sodium dodecyl sulfate solution over 12 hours yielded a fully decellularized construct, they report in the journal Nature Medicine.
The researchers reseeded such constructs with rat neonatal cardiac cells and aortic endothelial cells in a bioreactor that recreated cardiac physiology, with simulated coronary perfusion.
The perfused organ culture was maintained for between 8 and 28 days. By day 8 after seeding, the heart constructs showed electrical and contractile responses to single paces.
Under physiological load, the heart constructs produced a contractile force of around 2.4 mmHg, equivalent to approximately 2% of adult rat heart function, and 25% of 16-week-old fetal human heart function.
The researchers are optimistic that the technology could help to increase the donor organ pool. The supply of donor hearts for end-stage heart failure patients to undergo heart transplantation is limited, and once patients receive a heart they face lifelong immunosuppression, often trading heart failure for hypertension, diabetes, and renal failure, they explain.
"We used immature heart cells in this version, as a proof of concept. We pretty much figured heart cells in a heart matrix had to work," Taylor commented. "Going forward, our goal is to use a patient's stem cells to build a new heart."
Nature Med 2008; Advance online publication
# posted by roodbont @ 12:34 AM 
